Unanticipated Mycobacterium tuberculosis complex culture inhibition by immune modulators, immune suppressants, a growth enhancer, and vitamins A and D: clinical implications

Unanticipated Mycobacterium tuberculosis complex culture inhibition by immune modulators, immune suppressants, a growth enhancer, and vitamins A and D: clinical implications

Blog Article

Background: The development of novel antibiotics to treat multidrug-resistant (MDR) tuberculosis is time-consuming and expensive.Multiple immune modulators, immune suppressants, anti-inflammatories, and growth enhancers, and vitamins A and Oval Platter D, inhibit Mycobacterium avium subspecies paratuberculosis (MAP) in culture.We studied the culture inhibition of Mycobacterium tuberculosis complex by these agents.

Methods: Biosafety level two M.tuberculosis complex (ATCC 19015 and ATCC 25177) was studied in radiometric Bactec or MGIT culture.Agents evaluated included clofazimine, methotrexate, 6-mercaptopurine, cyclosporine A, rapamycin, tacrolimus, monensin, and vitamins A and D.

Results: All the agents mentioned above caused dose-dependent inhibition of the M.tuberculosis complex.There was no inhibition by the anti-inflammatory 5-aminosalicylic acid, which causes bacteriostatic inhibition of MAP.

Conclusions: We conclude that, at a minimum, studies with virulent M.tuberculosis are indicated with the agents mentioned White Willow Bark above, as well as with the thioamide 5-propothiouricil, which has previously been shown to inhibit the M.tuberculosis complex in culture.

Our data additionally emphasize the importance of vitamins A and D in treating mycobacterial diseases.